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Indonesian Journal of Agriculture

Indonesian Journal of Agricultural Science

Jurnal Penelitian dan Pengembangan Pertanian
Jurnal Perpustakaan Pertanian
Buletin Teknik Pertanian

Majalah Pengembangan Inovasi Pertanian

Warta Penelitian dan Pengembangan Pertanian
 


 
 

PUBLIKASI BADAN LITBANG PERTANIAN


>>Publikasi Elektronis | Jurnal Bioteknologi Pertanian | Volume. 9 No. 2 Th. 2004

Isolasi dan karakterisasi gen pengaktif transkripsi antosianin dari pustaka cDNA ubi jalar

Judul

:

Isolasi dan karakterisasi gen pengaktif transkripsi antosianin dari pustaka cDNA ubi jalar

Penulis

:

Muchdar Soedarjo; Koshun Ishiki.

Jurnal

:

Jurnal Bioteknologi Pertanian

Volume

:

9

Nomor

:

2

Tahun

:

2004

Halaman

:

p. 33-40.

Bahasa

:

Indonesian

Summary

:

Temporal and spatial biosyntheses of anthocyanin in various plant tissues are coordinately controlled by the expression of anthocyanin transcriptional activator genes (ATAG). So far, ATAG has not been extensively studied on sweet potato in spite of its high anthocyanin content. A study was undertaken to isolate and characterize the ATAG from the purple-fleshed tuberous root of sweet potato. The myb type ATAG fragment of Perilla frutescens (ao-jiso) was used as a probe to isolate the myb type ATAG from the cDNA library of the purplefleshed tuberous root of sweet potato. Fourteen positive plaques were detected from the cDNA library. Two clones out of the 14 clones revealed homology to the myb type transcriptional activator gene, including the myb type ATAG in maize. The nucleotide sequence of the two clones were the same, hence, they belonged to the same clone. The clone was named as sp4 and had a size of 1253 bp. Putative coding sequence started from nt. 97 to nt. 933. Thus the clone had 278 amino acid residues. The clone contained two DNA binding domain at its N terminal and acidic region at carboxyl terminal, which were the features commonly observed in the myb type ATAG. Therefore, the clone was probably a transcriptional regulatory gene of anthocyanin biosynthesis in sweet potato.

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